Coding

Part:BBa_K5301009:Design

Designed by: Jiaxin Guan   Group: iGEM24_BNU-China   (2024-09-22)


MCherry [11] is a part of bimolecular fluorescence complementation (BiFC) system.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

It should be codon-optimized for expression in the certain chassis.

Source

It was developed by splitting mCherry, a mutant monomeric red fluorescent protein, into two fragments. It is based on sfmcherry1. And that sequence is from Bo Huang's lab.

References